It seems the more we find out, the less we actually know.

Over the last week Ben and I have been running experiments almost nonstop. We are testing DCQ at pH 6.2, 7.4, and 9.0. In addition to that we have one set in just water and the other with hydrogen peroxide to see if DCQ reacts with H2O2 when it is supposed to just react with HRP to react with TCP. Each run on the HPLC takes about four hours to run usually. But as predicted, the DCQ at pH 9.0 with H2O2, the reaction went incredible fast and DCQ was gone after 21 minutes. I was worried that it would be to fast to even get a reading in the HPLC at all.
There is definitely a some evidence here of pH dependency but it's not at all what we thought it would be. Theoretically pH 6.2 should be the most stable pH but it doesn't appear to be that way. So now it is exactly like the title of this blog. And we have to take a step back and look at this and know when to stop and publish something then continue. But for the time being the HPLC is keeping pretty busy and will be for the rest of the week.