Data, Data, Data

This week has been an extremely busy week for us. I have doing a lot on the HPLC this week and I am know Ben has been running equations and the UV-vis as well.
After reading a journal article me and Brad decided to put our reaction of TCP + H2O2+ HRP to DCQ + (x) into di-ethyl either to stop the reaction in its place. But after a sort of failed attempt we managed to get a little bet of solution so we ran it in the HPLC and saw a peak when we stopped the reaction at about 10 seconds. This was interesting to see a new peak in something I thought I was starting to get a hold on. But because of the screw up and uncertainty of the data, we decided to try it again but with some slight changes and would be more prepaired this time.
So we raised the pH from 5 up to 7.4 so slow the reaction down some and we decided to take an allaquat every 30 seconds and put it into di-ethyl ether. In total we had eleven samples (one with TCP in dEE and no enzyme) and two standards. Of the eleven samples there were two things in the viles that were both going to be analyzed. The di-ethyl ether and the aquous solution at the bottom. So one by one I had to go through and extract what I needed and put them into viles and get them ready to be put in the HPLC. So now I have 24 different samples to run and we decide to run them twice. So I had to go through the HPLC and name 50 injections individually. And since there are two channels for each injection I have 100 things to look at and analyze. Not that you care I just want you to know how long and hard this is haha.
So the rest of the day and probably a few days next week will be spent looking at everything from one day of work. But hey, that's research.